Biology Transpiration Practical - 1603 Words

An experiment proving transpiration in leaves Aim To see how different environmental conditions affect transpiration of a leaf and which side of the leaf transpires the most. Background Information Plants put down roots into the soil to draw water and nutrients up into the stems and leaves. Some of this water is returned to the air by transpiration (when combined with evaporation, the total process is known as evapotranspiration). Transpiration rates vary widely depending on weather conditions, such as temperature, humidity, sunlight availability and intensity, precipitation, soil type and saturation, wind, land slope, and water use and diversion by people. During dry periods, transpiration can contribute to the loss of moisture in the†¦show more content†¦The water will not be able to evaporate. There are though some stomata on the upper side and there could be some water loss (evaporation). * Top Vaseline – There will be a significant amount of water loss. Most of the stomata are located on the underside of the leaf, and that hasn’t been covered. The upper surface does have some stomata, but not very much. * Whole Vaseline – This leaf will have t he least amount of water loss. Every single stoma on the leaf has been covered. The lipids in the Vaseline should stop the water from evaporating. Therefore, in order of 1-4 (1 – lost most water, 4 – lost least water), in terms of water lost – 1. Control 2. Top Vaseline 3. Bottom Vaseline 4. Whole Vaseline Apparatus * White tile * Figure [ 1 ] 4 leafs (similar size and type) * Vaseline * Weight scale * 2 stands * Thread * Labels * Tissue napkins Method 1. Gather your 4 leaves. 2. Take two stands and tie a thread (as shown in the Figure 1) 3. Take each leaf, put the Vaseline, then measure the mass and then hang it onto the thread. a. Leaf A – Control; No Vaseline used. Simply take mass and hang onto thread. b. Leaf B – Vaseline only on the top (and stem). 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